| Description |
All experiments are correlated to Nester's Microbiology: A Human Perspective, 5/e, but can be used with any non-majors/allied health microbiology textbook. |
| Key Features |
The exercise involving the identification of Gram negative organisms (Exercise 24) has been expanded . The controls in the transformation experiment (Exercise 16) are no longer explained, forcing the student to analyze them more carefully . The Ribosomal Data Project directions (Exercise 35) have been updated to reflect the changes of the web site (Michigan State online analysis -- http://rdp.cme.msu.edu). Alternate procedures: Some instructors requested other procedures for making plate counts, calculating dilutions, and increased data collecting opportunities for graphing. These procedures were included, but in such a way that they were optional for an instructor who preferred the original methods. The safety of certain organisms was carefully considered and some organisms were removed that might pose a potential hazard even though there have not been problems in the past. A bibliography of books related to microbiology was added to the appendix which should be interesting to students that now have a background in microbiology.
|
| Table of Contents |
Part One: Basic Microbiology Introduction to Microbiology Exercise: 1 Ubiquity of Microorganisms Exercise: 2 Bright-Field Light Microscopy, Including History and Working Principles Exercise: 3 Microscopic (Bright-Field and Dark-Field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations Introduction to Staining of Microorganisms Exercise: 4 Simple Stains: Positive and Negative Stains Exercise: 5 Differential and Special Stains Introduction to Microbial Growth Exercise: 6 Pure Culture and Aseptic Technique Exercise: 7 Chemically Defined, Complex, Selective, and Differential Media Exercise: 8 Quantification of Microorganisms Introduction to the Environment and Microbial Growth Exercise: 9 Aerobic and Anaerobic Growth Exercise: 10 The Effect of Incubation Temperature on Generation Time Introduction to Control of Microbial Growth Exercise: 11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time Exercise: 12 Control of Microbial Growth with Ultraviolet Light Exercise: 13 Osmotic Pressure, and Its Effect on Rate and Amount of Microbial Growth Exercise: 14 Antiseptics and Antibiotics Introduction to Microbial Genetics Exercise: 15 Selection of Bacterial Mutants Resistant to Antibiotics Exercise: 16 Transformation: A Form of Genetic Recombination Exercise: 17 Bacterial Conjugation Exercise: 18 Gene Regulation: Induction and Catabolite Repression Part Two: The Other Microbial World Introduction to the Other Microbial World Exercise: 19 Microscopic Identification of Fungi Exercise: 20 Parasitology: Protozoa and Helminths Exercise: 21 Titering Prokaryotic Viruses Part Three: Microbes and Humans Introduction to Medical Microbiology Exercise: 22 Normal Skin Flora Exercise: 23 Respiratory Microorganisms Exercise: 24 Identification of Enteric Gram-Negative Rods Exercise: 25 Clinical Unknown Identification Part Four: Immunology Introduction to Some Immunological Principles and Techniques Exercise: 26 Differential White Blood Cell Stains Exercise: 27 Lysozyme, an Enzymatic Form of Natural Resistance Exercise: 28 Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test Exercise: 29 Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides Immitis Identification Exercise: 30 An Ouchterlony Double Immunodiffusion Test for Coccidioides Immitis Identification Part Five: Public Health Introduction to the Prevention and Control of Communicable Diseases Exercise: 31 Epidemiology: A Staphylococcus Carrier Study Exercise: 32 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques Part Six: Applications of Biotechnology Introduction to Biotechnology Exercise: 33 Identifying DNA with Restriction Enzymes Exercise: 34 Identification of Bacteria Using the Ribosomal Data Project Part Seven: Projects Introduction to the Individual Projects Exercise: 35 Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills Exercise: 36 Luminescent Bacteria: Bacteria That Produce Light Exercise: 37 Methylotrophs, Organisms That Grow on One-Carbon Compounds Exercise: 38 Deinococcus, Bacteria with Out-Of-This-World Capabilities Appendices 1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual 2. Dilution Practice Problems 3. Metric System, Use of with Conversions to the English System of Measurement 4. Alternative Procedures 5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size 6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension 7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus 8. Additional Reading |
